Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages and dendritic cells. Its main role is to attract monocytes, basophils and T cells. CCL2 is involved in pathologies involving monocytic infiltrates like psoriasis or atherosclerosis.In the brain, CCL2 is released by neurons, astrocytes and microglia and is part of the neuroinflammatory response observed in epilepsy, ischemia or Alzheimer’s disease.CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to Diabetes.
Cisbio's Human CCL2 kit is a homogeneous, add and read assay to measure CCL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||20 pg/mL & 90 pg/mL|
|Range||90 - 3,000 pg/mL|
|Time to result||ON at RT|
|Calibration||NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL)|