Human CCL2 (MCP1) Assay Kit

Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages and dendritic cells. Its main role is to attract monocytes, basophils and T cells. CCL2 is involved in pathologies involving monocytic infiltrates like psoriasis or atherosclerosis.In the brain, CCL2 is released by neurons, astrocytes and microglia and is part of the neuroinflammatory response observed in epilepsy, ischemia or Alzheimer’s disease.CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to Diabetes.
Cisbio's Human CCL2 kit is a homogeneous, add and read assay to measure CCL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 20 pg/mL & 90 pg/mL
Range 90 - 3,000 pg/mL
Time to result ON at RT
Calibration NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL)
Species Human only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Add

Cytokines assay principle add

Read

Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run CCL2 analysis.

 

How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance

Precision

Intra-assay (n=24)

Sample Mean [CCL2]
(pg/mL)
CV
1 100 10%
2 1500 7%
3 2750 7%
  Mean CV 8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [CCL2]
(pg/mL)
Mean (delta R) CV
1 121 335 2%
2 437 1096 3%
3 1579 3030 2%
    Mean CV 2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human CCL2 kit - 500 tests62HCCL2PEG
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Human CCL2 kit - 10,000 tests62HCCL2PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human CCL2 kit - 3 Standards62HCCL2CD3
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Human CCL2 kit - Standard62HCCL2CDA
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