Immune checkpoint assay to identify CTLA4/B7-2 (CD86) checkpoint inhibitors
CTLA4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is an inhibitor of the immune response expressed by T cells.
CTLA4 and CD28 (stimulatory checkpoints) share the same ligands, namely CD80 (B7-1) and CD86 (B7-2), with CTLA4 displaying a greater affinity than CD28 for both and thus creating effective ligand binding competition.
While CTLA4 can be found on all T cells, it is mainly expressed on Treg where it participates in their inhibition of the immune response.
The HTRF CTLA4/B7-2 Binding Assay is designed to measure the interaction between Human CTLA4 and B7-2 (CD86) proteins. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and anti-human antibody inhibitors in a high throughput format.
Our kits include everything you need to set up your biochemical test: validated recombinant proteins, labelled detection antibodies, and buffers. They can be used in all formats from 96 to 1536 wells, and have been validated with reference antibodies and compounds.