Human CTLA4 / B7-2 (CD86) biochemical interaction assay

Immune checkpoint assay to identify CTLA4/B7-2 (CD86) checkpoint inhibitors

CTLA4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is an inhibitor of the immune response expressed by T cells.

CTLA4 and CD28 (stimulatory checkpoints) share the same ligands, namely CD80 (B7-1) and CD86 (B7-2), with CTLA4 displaying a greater affinity than CD28 for both and thus creating effective ligand binding competition.

While CTLA4 can be found on all T cells, it is mainly expressed on Treg where it participates in their inhibition of the immune response.

The HTRF CTLA4/B7-2 Binding Assay is designed to measure the interaction between Human CTLA4 and B7-2 (CD86) proteins.  Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and anti-human antibody inhibitors in a high throughput format.

Our kits include everything you need to set up your biochemical test: validated recombinant proteins, labelled detection antibodies, and buffers. They can be used in all formats from 96 to 1536 wells, and have been validated with reference antibodies and compounds.

Download CTLA4/B7-2  (CD86) biochemical technical note

Assay principle

HTRF® - The homogeneous biochemical immunoassay

Each kit includes recombinant immune checkpoint partners tagged for detection.

Without treatment, the partners interact and the binding of each detection antibody to its tag target generates an HTRF signal.

Product performances

Inhibitory effects of blocking mAb

 The inhibitory effects of Mouse anti-human CTLA4 were tested at 6nM CTLA4 and 6 nM B7-7. An assay window around 2 was obtained.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
CTLA4/B7-2 binding assay kit - 500 tests64ICP05PEG
CTLA4/B7-2 binding assay kit- 10,000 tests64ICP05PEH