Human FMRP Assay Kit

HTRF® assay kit for detecting FMRP, a critical protein in fragile X syndrome

HTRF® FMRP assay is a rapid sandwich immunoassay for accurate quantification of the endogenous level of the fragile X mental retardation 1 protein in cell lysates. Based on our HTRF® technology, the straightforward assay protocol does not need any wash steps. The assay is suitable for miniaturization and enables work with low sample volumes. The FMRP ready-to-use kit contains all the reagents you need and offers enhanced convenience over ELISA and Western Blot. The FMRP assay kit is designed for CNS research, for fragile X syndrome, premature ovarian failure, autism and Parkinson's disease.

Assay principle

Human FMRP is measured using sandwich immunoassays involving two specific anti-FMRP antibodies, respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the FMRP present in the sample.

The human FMRP assay protocol is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human FMRP by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human FMRP kit - 500 tests63ADK038PEG
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Human FMRP kit - 10,000 tests63ADK038PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human FMRP kit - control lysate63ADK038TDA
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