Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce their mobilization in the blood stream, and on neurons to promote neurogenesis and block apoptosis.
In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery.
The absence of a functional G-CSF receptor is associated with congenital neutropenia
Cisbio's Human G-CSF kit is a homogeneous, add and read assay to measure G-CSF secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||27 pg/mL & 47 pg/mL|
|Range||47 – 4,500 pg/mL|
|Time to result||Overnight at RT|
|Calibration||NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF|