Type I interferons, interferon alpha (IFN-α) and interferon beta (IFN-β), play essential roles in the innate immune response to viral infections. IFN-β is produced by fibroblasts, macrophages, and dendritic cells, as well as endothelial cells. The expression of the IFN-β gene is controlled by transcription factors like IRF-3, c-Jun/ATF-2, and NF-kappaB downstream of Pattern Recognition Receptors, such as Toll-like receptors. In particular, IFN-β is the primary functional readout of cGas-cGAMP-STING pathway activation. Once secreted, IFN-β binds and activates the IFNAR1/IFNAR2 receptor complex, which relies on STAT1/2 transcription factors. Phosphorylated STAT1/2 leads to the induction of anti-viral gene expression.
Cisbio's Human IFN-β kit is a homogeneous add and read assay to measure IFN-β secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||14 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, activation reagent, buffers & protocol|
|LOD & LOQ (in Diluent)||7 pg/mL & 19 pg/mL|
|Range||19 – 4,000 pg/mL|
|Time to result||3h @ RT|
|Calibration||NIBSC (00/572) value (IU/ml) = 0.44 x HTRF hIFNβ value (pg/ml)|