Human IL1 alpha Assay Kit

Also known as hematopoietin 1, IL1α is a pro-inflammatory cytokine mainly produced by activated macrophages, epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1α stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.
Cisbio's Human IL1α kit is a homogeneous, add and read assay to measure IL1α secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 8 pg/mL & 20.5 pg/mL
Range 20.5 – 5,000 pg/mL
Time to result 2h at RT
Calibration NIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1α value (pg/mL)
Species Human only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.


Cytokines assay principle add


Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run IL1α analysis.


How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance


Intra-assay (n=24)

Sample Mean [IL1α] (pg/mL) CV
1 50 11%
2 2500 2%
3 4500 4%
  Mean CV 6%

Each of the 3 samples was measured 24 times, and % CV iwas calculated for each sample.

Inter-assay (n=4)

Sample [IL1α] (pg/mL) Mean (delta R) CV
1 78 246 6%
2 411 1298 7%
3 2174 5964 7%
    Mean CV 7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human IL1 alpha kit - 500 tests62HIL1APEG
Human IL1 alpha kit - 10,000 tests62HIL1APEH

Companion products

DescriptionCat. noProduct insertMSDS
Human IL1 alpha kit - 3 Standards62HIL1ACD3
Human IL1 alpha kit - Standard62HIL1ACDA