Also known as CSIF (Cytokine synthesis inhibitory factor), IL10 is an anti-inflammatory cytokine. Its negative regulation of the immune response is due to its ability to block the signaling of other cytokines, like IFNg, and to inhibit CD28 phosphorylation in T cells.
In immuno-oncology, IL10 is known to inhibit the expansion of TH1 cells while promoting the development of Treg and macrophages.
Cisbio's Human IL10 kit is a homogeneous, add and read assay to measure IL10 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||7 pg/ml & 22 pg/mL|
|Range||22 – 6,000 pg/mL|
|Calibration||NIBSC (93/722) value (IU/mL) = 0,005 x HTRF hIL10 value (pg/mL)|
|Time to result||Overnight at RT|