Human IL17 Assay Kit

IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of the response to fungi and bacteria, and promotes the release of other pro-inflammatory cytokines and chemokines.
Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and auto-immune diseases like asthma, psoriasis or IBD (inflammatory bowel disease).
In immuno-oncology, IL17 is the main cytokine released by differentiated TH17 cells
Cisbio's Human IL17 kit is a homogeneous, add and read assay to measure IL17 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 7 pg/mL & 33 pg/mL
Range 33 – 10,000 pg/mL
Time to result Overnight at RT
Calibration NIBSC (01/420) value (IU/mL) = 0,02 x HTRF hIL17 value (pg/mL)
Species Human only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Add

Cytokines assay principle add

Read

Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run hIL17 analysis.

 

How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance

Precision

Intra-assay (n=24)

Sample Mean [IL17] (pg/mL) CV
1 114 6%
2 4644 2%
3 8634 2%
  Mean CV 3%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL17] (pg/mL) Mean (delta R) CV
1 194 350 6%
2 939 1848 11%
3 4545 8485 3%
    Mean CV 7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human IL17 kit - 500 tests62HIL17PEG
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Human IL17 kit - 10,000 tests62HIL17PEH
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Human IL17 kit - 1 x 96 tests62HIL17PET
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Companion products

DescriptionCat. noProduct insertMSDS
Human IL17 kit - 3 Standards62HIL17CD3
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Human IL17 kit - Standard62HIL17CDA
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