Immune checkpoint assay to identify LAG3/MHC II checkpoint inhibitors
The lymphocyte activation gene-3 (LAG-3), selectively transcribed in human activated T and NK cells, encodes a ligand for major histocompatibility complex (MHC) class II molecules. When LAG3 is bound to MHC II, T cell response is suppressed. Checkpoint inhibitors blocking LAG3/MHC II complex formation are generating considerable interest in cancer immunotherapy.
The HTRF LAG3/MHC II Binding Assay is designed to measure the interaction between Human LAG3 and MHC II proteins. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and anti-human antibody inhibitors in a high throughput format.
Our kits include everything you need to set up your biochemical test: validated recombinant proteins, labelled detection antibodies, and buffers. They can be used in all formats from 96 to 1536 wells, and have been validated with reference antibodies and compounds.