Human MBNL1 Assay Kit

HTRF® assay kit for detecting MBNL1 in cell lysates

Human MBNL1 kit is a sandwich immunoassay for rapid and accurate quantification of the muscle blind-like protein 1 in cell lysates. The simple, robust add & read procedure means increased throughput compared to ELISAs. Only a low volume of sample is required, which saves costly biological material.

Assay principle

Human MBNL1 is measured using a sandwich immunoassay involving two specific anti-MBNL1 antibodies respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the human MBNL1 present in the sample.

The human MBNL1 assay protocol is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human MBNL1 by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human MBNL1 kit - 500 tests63ADK012PEG
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Human MBNL1 kit - 10,000 tests63ADK012PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human MBNL1 kit - Standard63ADK012CDA
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