Immune checkpoint assay to identify PD1/PD-L1 checkpoint inhibitors
Programmed cell death protein 1 (PD1) is an immune checkpoint receptor that regulates T cell response. Its ligand, programmed death-ligand 1 (PD-L1), is commonly over-expressed on the tumor cell surface. When PD1 is bound to PD-L1, T cell response is suppressed, contributing to tumor immune resistance. Checkpoint inhibitors blocking PD1/PD-L1 complex formation are generating considerable interest in cancer immunotherapy.
The HTRF PD1/PD-L1 Binding Assay is designed to measure the interaction between Human PD1 and PD-L1 proteins. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody inhibitors in a high throughput format. This kit includes human PD1 and PD-L1 proteins and is designed to test anti-human antibody blockers or small molecules
Our kits include everything you need to set up your biochemical test: validated recombinant proteins, labelled detection antibodies, and buffers.They can be used in all formats from 96 to 1536 wells, and have been validated with reference antibodies and compounds.