Human PD1/PD-L1 biochemical interaction assay

Immune checkpoint assay to identify PD1/PD-L1 checkpoint inhibitors

Programmed cell death protein 1 (PD1) is an immune checkpoint receptor that regulates T cell response.  Its ligand, programmed death-ligand 1 (PD-L1), is commonly over-expressed on the tumor cell surface.  When PD1 is bound to PD-L1, T cell response is suppressed, contributing to tumor immune resistance.  Checkpoint inhibitors blocking PD1/PD-L1 complex formation are generating considerable interest in cancer immunotherapy.

The HTRF PD1/PD-L1 Binding Assay is designed to measure the interaction between Human PD1 and PD-L1 proteins.  Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody inhibitors in a high throughput format. This kit includes human PD1 and PD-L1 proteins and is designed to test anti-human antibody blockers or small molecules

Our kits include everything you need to set up your biochemical test: validated recombinant proteins, labelled detection antibodies, and buffers.They can be used in all formats from 96 to 1536 wells, and have been validated with reference antibodies and compounds.

Download PD1/PD-L1 technical note
Read scientific paper to assess PD-L1 binder specificity for immunotherapy follow-up

Assay principle

HTRF® - The homogeneous biochemical immunoassay

Each kit includes recombinant immune checkpoint partners tagged for detection.

Without treatment, the partners interact and the binding of each detection antibody to its tag target generates an HTRF signal.

Product performances

Inhibitory effects of PD1/PD-L1 blockers

The inhibitory effects of small molecules, human and mouse anti-human blocking antibodies of PD1 and PD-L1 were tested at 5 nM PD-L1 and 50 nM PD1. An assay window of 10-fold was obtained. 

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
PD1/PD-L1 binding assay kit - 500 tests64ICP01PEG
PD1/PD-L1 binding assay kit - 10,000 tests64ICP01PEH