TNFα is a chemokine mainly produced by macrophages, T cells and neutrophils. TNFα acts as a chemoattractant for neutrophils, and promotes infiltration and activation at inflammation sites.
TNFα is involved in several cancer types for its ability to promote angiogenesis and cell proliferation, as well as inhibit apoptosis.
Cisbio's Human TNFα kit is a homogeneous, add and read assay to measure TNFα secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||5 pg/mL & 29 pg/mL|
|Range||29 – 2,500 pg/mL|
|Time to result||2h at RT|
|Calibration||NIBSC (12/154) value (IU/mL) = 0,1 x HTRF hTNFα value (pg/mL)|