Human TREM-2 Assay Kit

Sandwich immunoassay kit designed for the rapid detection of TREM-2

Based on the TR-FRET homogeneous robust HTRF® technology, the kit is designed for the rapid detection of TREM-2 (also known as Triggering Receptor Expressed on Myeloid Cells 2) in cell supernatant and whole cells. This assay enables high throughput without any wash steps, reducing assay time compared to ELISA. HTRF’s simple protocol ensures quick assay times, with results obtained after overnight incubation. This procedure is suitable for miniaturization and allows low sample sizes.  By detecting TREM-2, this HTRF® kit is a key tool for neurodegeneration research, especially Alzheimer’s disease.

 

Assay principle

HTRF® - the homogeneous sandwich immunoassay

Human TREM2 is measured using sandwich immunoassays involving two specific anti-human TREM2 antibodies, respectively labeled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the TREM2 present in the sample.

Assay protocol

The simple TREM2 assay protocol, using a 384-well small volume white plate (20 µL final), is described on the right. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection of human TREM2 by HTRF® reagents. The antibodies labeled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally

Product performance

Detection of TREM-2 on THP-1 Cell Supernatant

 

THP1 cells were differentiated by adding 25 µM of PMA, causing cells to become adherent.  The stimulation was done during 3 days.  Once attached the cells were allowed to recover in fresh RPMI+10%FBS for 24 hours.  Cell supernatant was collected after 24 hours and tested in the assay.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human TREM-2 kit - 500 tests63ADK099PEG
-
Human TREM-2 kit - 10,000 tests63ADK099PEH
-

Companion products

DescriptionCat. noProduct insertMSDS
Human TREM-2 kit - standard63ADK099CDA
-
-

ドキュメント