Hyaluronic Acid Assay Kit

Sandwich immunoassay kits designed for the rapid detection of Hyaluronic acid

Based on our TR-FRET homogeneous and robust HTRF® technology, the Hyaluronic acid sandwich immunoassay kits are designed for the rapid detection of Hyaluronic acid in cell supernatant. This simple and robust procedure is suitable for miniaturization and enables work with low sample sizes. The add & read protocol eliminates all wash steps for faster analysis. By detecting Hyaluronic acid, this HTRF® kit is a critical tool for research related to liver fibrosis.

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Assay principle

HTRF® - the homogeneous sandwich immunoassay

Hyaluronic acid is measured using sandwich immunoassays involving Hyaluronic Acid Binding Protein (HABP) labeled with terbium cryptate (donor) and a HABP labeled with d2 (acceptor). The intensity of the signal is proportional to the concentration of the Hyaluronic acid present in the sample.

ASSAY PROTOCOL

The Hyaluronic acid assay protocol is described here. Cells are plated, stimulated in the same 96-well culture plate. Cell supernatants are then transferred to the assay plate for the detection of Hyaluronic acid by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The binding buffer is required to enhance the detection of hyaluronic acid on samples. The HABP labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Hyaluronic acid kit - 500 tests63ADK089PEG
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Hyaluronic acid kit - 10,000 tests63ADK089PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Hyaluronic acid kit - Standard63ADK089TDA
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