Myosin Heavy Chain Assay Kit

Sandwich immunoassay kits designed for the rapid detection of Myosin Heavy Chain 

Based on our TR-FRET homogeneous and robust HTRF® technology, the Myosin Heavy Chain sandwich immunoassay kits are designed for the rapid detection of Myosin Heavy Chain in cell lysates. This simple and robust procedure is suitable for miniaturization and enables work with low sample sizes. The add & read protocol eliminates all wash steps for faster analysis. By detecting Myosin Heavy Chain, this HTRF® kit is a critical tool for research related to skeletal muscle diseases.

Assay principle

HTRF® - the homogeneous sandwich immunoassay

Myosin Heavy Chain is measured using sandwich immunoassays involving two specific anti-Myosin Heavy Chain antibodies, respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the Myosin Heavy Chain present in the sample

ASSAY PROTOCOL

The Myosin Heavy Chain assay protocol is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Myosin Heavy Chain by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Product performance

Detection of Myosin Heavy Chain

 

C2C12 cells were grown in presence of different percentage of Fetal Bovine Serum (FBS) or Horse Serum (FS) to induce differentiation of C2C12 myoblasts into myotubes. After 4 or 8 days  incubation, media was aspirated and cells were lysed with lysis buffer 1X for 30 min at RT under gentle shaking. 16µL of lysate were transferred into a 384-well sv white microplate and 4 µL of the HTRF Myosin Heavy Chain detection reagents were added. The HTRF signal was recorded after a overnight incubation at room temperature.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Myosin Heavy Chain kit - 500 tests63ADK098PEG
-
Myosin Heavy Chain kit - 10,000 tests63ADK098PEH
-

Companion products

DescriptionCat. noProduct insertMSDS
Myosin Heavy Chain kit - control lysate63ADK098TDA
-
-