Phospho- and total CREB assays for monitoring CREB pathway
Cisbio's cell-based homogeneous HTRF® total CREB assay kit is designed for the quantitative detection of the cyclic AMP-responsive element-binding protein, enabling the phosphorylation status of CREB to be checked with respect to its steady-state level in cells. Ideal for CREB normalization, the total CREB kit is compatible with the buffers from the phospho-CREB kit, so the same lysate can be used for analyses of both the phosphorylated and the total protein populations. CREB functions as a transcription factor, signaling downstream from MAPK/ERK activated by several pathways, such as MAPK/ERK, PI3K/AKT and stress signaling. This means the protein plays a key role in many biological processes, for example promoting neuronal plasticity, cognitive abilities, cell proliferation, survival and glucose homeostasis. The HTRF® total-CREB assay is an ideal tool for oncology, CNS, diabetes and inflammation research.