HTRF® cell-based phospho and total-Histone H3 assays for anti-cancer research
The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. Phosphorylation of Ser10 or Thr3 in the tails of Histone H3 is involved in gene expression and during mitosis.
Phosphorylation at Ser10 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Phosphorylation at Threonine 3 catalyzed by the kinase haspin and highly conserved among many species leads to recruiting Aurora kinase B (AURKB) and the chromosomal passenger complex (CPC) to kinetochores, to regulate mitosis.
Cisbio's cell-based homogeneous phospho-Histone H3 assay kits are designed for the cell-based quantitative detection of phosphorylation on Histone H3. The HTRF® total Histone H3 assay kit is designed for the quantitative detection of total Histone H3, phosphorylated and unphosphorylated, to normalize the phosphorylation status of Histone H3.
The buffers of both HTRF® phospho- and total Histone H3 assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample.