Phospho- and total Insulin Receptor assays for IR detection
Cisbio's cell-based homogeneous kit for phospho-Insulin receptor (Tyr1150/1151) enables the quantitative detection of Insulin Receptor modulation, phosphorylated at Tyrosines 1150 & 1151. The HTRF® total Insulin Receptor assay kit is designed for the quantitative detection of total Insulin Receptor, phosphorylated and unphosphorylated, to normalize the phosphorylation status of Insulin Receptor with respect to its steady-state level in cells. The buffers of both HTRF® phospho- and total IR assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample. Stimulated by insulin and insulin-like growth factors, the insulin receptor is involved in several pathways such as those for Insulin and FoxO signaling. This tyrosine kinase receptor acts as one of the major receptors in glycemia regulation, and is a pertinent protein in the diabetes and metabolism research areas. The simple add-and-read protocol eliminates all wash steps for a faster analysis and easy miniaturization, while maintaining a high-quality specific output and offering enhanced convenience over ELISA or WB.