Alpha-SMA kit
Alpha-SMA quantification in cell lysates
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This HTRF cell-based assay enables rapid, quantitative detection of SMAD1 phosphorylated at Serine 463/465. After phosphorylation and translocation to the nucleus, SMAD1 acts as a transcription factor in regulating the expression of genes involved in stem cell renewal, cell proliferation, apoptosis, migration, differentiation, and immune responses.
Mouse C2C12 cells were cultured to 80% confluency. After BMP-4 treatment, cells were lysed and soluble supernatants were collected via centrifugation. Serial dilutions of the cell lysate were performed and 16 µL of each dilution were transferred into a 384-well low volume white microplate before finally adding phospho kit reagents. A side by side comparison showed the HTRF Phospho assay is at least 8-fold more sensitive than the Western Blot, and shows optimal correlation.
BMP/SMAD signaling pathway plays a key role in embryogenesis, development, adult tissue homeostasis and immune responses. It is mediated by heterotetrameric complexes of BMPRI and BMPRI, that activate the intracellular signaling proteins SMAD1/5/8 by phosphorylating two C-terminal serine residues.
BMP-2/4 binding on BMPRI triggers the recruitment of BMPRII, which then transphosphorylates the intracellular domain of BMPRI.
BMPRI in turn phosphorylates SMAD1 at Ser463 and Ser465, enabling its oligomerization with the co-mediator (Co-SMAD) SMAD4. This complex translocates to the nucleus, where it functions as a transcription factor with coactivators and corepressors to regulate gene expression.
Inhibitory SMAD6 and SMAD7 are involved in feedback inhibition of the signaling pathway.
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Insight into fibrosis development and signaling pathways
A comprehensive overview of fibrosis development - Guides
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Improving Immunotherapy by Targeting the Tumor Stroma - Guides
Manual SMAD1 P-S463/465 Kit / 63ADK062PEG-63ADK062PEH
63ADK062PEG-63ADK062PEH - Product Insert
Manual SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Product Insert
Safety Data Sheet (DEU) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (ELL) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (ITA) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (SPA) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG - Safety Data Sheet
Certificate of Analysis SMAD1 P-S463/465 Kit / 63ADK062PEG
63ADK062PEG Batch 09D - Quality Control Report
Safety Data Sheet (DEU) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (ELL) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (ITA) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (SPA) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) SMAD1 P-S463/465 Kit / 63ADK062PEH
63ADK062PEH - Safety Data Sheet
Safety Data Sheet (DEU) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (ELL) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (FRA-FR) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (ITA) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (SPA) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (ENG-GB) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Safety Data Sheet (ENG-US) SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET - Safety Data Sheet
Certificate of Analysis SMAD1 P-S463/465 Kit / 63ADK062PET
63ADK062PET Batch 01Q - Quality Control Report
Safety Data Sheet (DEU) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (ELL) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (ITA) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (SPA) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
Safety Data Sheet (ENG-US) SMAD1 P-S463/465 Kit / 63ADK062PEY
63ADK062PEY - Safety Data Sheet
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