Phospho- and total VASP assays for monitoring PKA and PKG activation
Cisbio's cell-based homogeneous HTRF® phospho-VASP assays enable quantitative detection of the modulation of VASP phosphorylated on Serine 157 or Serine 239. In endothelial cells, VASP is phosphorylated by the PKA kinase on Serine 157 and by PKG kinase on Serine 239. It is involved in cell motility, migration, and adhesion. This makes phospho-VASP assays a valuable tool in cardiovascular, oncology and inflammation research.
Using a streamlined protocol without any washing steps and amenable to low-volume formats, the phospho-VASP assay can be used from basic research up to preclinical drug discovery phases. The kit contains all the reagents you need, and offers increased throughput compared to ELISA or Western Blot.