Rat IGF-1 Assay Kit

HTRF® assay kit for measuring rat IGF-1 in cell supernatants

The rat IGF-1 HTRF® kit (also known as somatomedin C and somatomedin A) is a rapid sandwich immunoassay optimized for the quantification of rat insulin-like growth factor 1 concentrations in cell supernatant. Developed with the add-and-read no-wash HTRF technology,  the kit represents a significant improvement over traditional ELISA, resulting in major time savings. The kit combines high detection performance with low sample consumption, making it the ideal solution for laboratories looking for a reliable and streamlined detection of rat IGF-1.

Assay principle

Rat IGF-1 is measured using a sandwich immunoassay with two antibodies specific for rat IGF-1, respectively labelled with Europium cryptate (donor fluorophore) and d2 (acceptor fluorophore). The intensity of the FRET signal is proportional to the concentration of the IGF-1 present in the sample.

The rat IGF-1 assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection of rat IGF-1 by HTRF® reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Rat IGF1 kit - 500 tests63ADK032PEG
-
Rat IGF1 kit - 10,000 tests63ADK032PEH
-

Companion products

DescriptionCat. noProduct insertMSDS
Rat IGF1 kit - Standard63ADK032CDA
-
-

ドキュメント