α-Synuclein aggregation Assay Kit

Cell-based assay for measuring alpha synuclein aggregation

Monitoring α-synuclein aggregation is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson’s disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA).

Cisbio Bioassays provides a solution to rapidly detect and quantify protein aggregates of human α synuclein in cell culture or brain tissue extracts.

The assay is built on a truly homogeneous protocol, with no need for washing or separation steps, thus increasing throughput and saving significant amounts of time compared to ELISA and filtration methods.

 

Assay principle

α-Synuclein aggregates are measured using a sandwich immunoassay involving an anti-α Synuclein monoclonal antibody specific for α Synuclein aggregate labeled with terbium-cryptate or d2, ensuring assay quality, reproducibility, and signal quality.

The specific HTRF signal generated is proportional to the α-Synuclein aggregates.

Product specifications

Measurement of alpha synuclein aggregation in human brain samples

Samples from human putamen (Parkinson’s versus control) were collected and lysed with Cisbio lysis buffer.

Due to the nature of the samples, the conventional cellular protocol needed to be adjusted. Incubation was performed for 20 hours at room temperature.

Even at high dilution (1/64), clear discrimination between the two sample types is possible with Cisbio’s aggregated alpha synuclein assay.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
A-Synuclein aggregation kit - 500 tests6FASYPEG
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A-Synuclein aggregation kit - 10,000 tests6FASYPEH
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