TGF beta 1, also known as Transforming Growth Factor beta 1, is a member of the transforming growth factor beta superfamily of cytokines. It contains more than 30 structurally related polypeptide growth factors including TGFβs (1–3).
TGF beta 1 is considered to be one of the major cytokines involved in the regulation of extracellular matrix (ECM) synthesis and degradation, as well as being a major profibrotic factor and important for wound healing. TGFβ superfamily ligands bind to a heteromeric receptor (TGFBR1/TGFBR2) which activates the SMADs signaling pathway. TGF beta 1 pre-proprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature TGF beta 1 peptide. The cleaved LAP and TGF beta 1 remain associated through strong non-covalent interactions. Thus, TGF beta 1 is present in biological fluids as a TGF beta1-LAP complex which requires an activation step to release the active form of TGF beta 1. This activation step is usually performed using an acid activation followed by a neutralization.
Cisbio's TGF beta 1 is a homogeneous, add and read assay to measure TGF beta 1 secretion in supernatants from human or murine cells. It offers a fast and efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Activation and neutralization reagents, lyophilized standard, frozen detection antibodies, buffers & protocol|
|LOD & LOQ (in Diluent)||4 pg/mL & 19 pg/mL|
|Range||19 – 2,000 pg/mL|
|Time to result||ON at RT|
|Calibration||NIBSC (89/514) value (U/mL) = 0.017 x HTRF hTGFβ value (pg/mL)|
|Species||Human, mouse, bovine (use of FCS is possible up to 5% max), others expected (based on sequences similarities).|
|Specificity||There is no cross-reactivity with human TGFβ2 and TGFβ3.|