-->

KRAS G12C GTP binding kit HTRF®

The KRAS G12C GTP binding kit is designed to identify KRAS G12C / GTP inhibitors.

See more
  • No-wash No-wash
  • All inclusive kit All inclusive kit
  • Accurate pharmacology Accurate pharmacology

The KRAS G12C GTP binding kit is designed to identify KRAS G12C / GTP inhibitors.

-

Overview

A fast and easy way to identify new KRAS G12C/GTP competitors.

KRAS is a small GTPase implicated in various biological processes, such as cell proliferation, cell survival, and cell metabolism. This proto-oncogene is well known to be mutated in many cancer subtypes, inducing an uncontrolled proliferation and cell metabolism modifications. It thereby contributes to the Warburg effect in cancer cells. Like the majority of small GTPases, KRAS binds to GDP in its inactive form or binds to GTP to switch into active form. KRAS G12C is one of the most commonly present mutant forms in cancer which leads to a permanently active state of KRAS. Identifying new KRAS/ GTP competitors is therefore a relevant strategy to control biological processes involved in cancer growth, by reducing the KRAS activity as well as the associated pathways.

Benefits

  • CANCER RESEARCH
  • KRAS INHIBITORS

GTP-Red reagent Kd

70nM

GTP-Red reagent concentration

50nM

KRAS GTP binding Standard (GDP) IC50

34nM

KRAS GTP binding Standard Ki

19.8nM

Screening of GTP competitors with human KRAS G12C protein

Various nucleotides known to be GTP competitors were added to the assay. GDP (assay standard), GTPyS, and GTP displayed the right potency, in good correlation with the literature. ATP, a non-specific nucleotide, and BAY-293, a KRAS/SOS1 inhibitor, had no effect on the assay, confirming the specificity of the kit.

Validation of the HTRF KRAS G12C GTP binding kit with nucleotides
Validation with competitive or non-competitive compounds

Best practices for pharmacological characterization of PPI inhibitors

Easy pharmacological characterization of PPI modulators. - Technical Notes

HTRF assays handle low- to high affinity protein-protein interactions

Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

HTRF PPI your dream assay served on a plate

Sandwiches aren't just for eating - Infographics

A brief history of Protein-Protein Interactions

How well do you know PPI? - Infographics

Virology research solutions using HTRF Protein-Protein Interaction assays

See how peer researchers challenge the viral life cycle with PPI assays - Application Notes

Product Insert KRAS G12C GTP Kit / 64BDKRASGPEG-64BDKRASGPEH

64BDKRASGPEG-64BDKRASGPEH - Product Insert

KRAS GTP competitors and KRAS/SOS1 inhibitors screening with new HTRF biochemical-based assays

Screening for KRAS competitors and inhibitors with HTRF technology - Application Notes

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

Let's find your reader

Latest news