Human CCL20 (MIP3 alpha) kit
No-wash kit to quantify released Human CCL20 (MIP3 alpha)
Leukotriene B4 kit HTRF®
The Leukotriene B4 kit is designed for the accurate quantification of LTB4.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
LTB4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. LTB4 is synthesized by leukocytes via the 5-lipoxygenase pathway and mediates different leukocyte functions such as lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTB4 kit enables the monitoring of LTB4 produced by cells and can be used to study the LTA4-hydrolase.
Benefits
- CELLS AND CELL SUPERNATANT FORMATS
- LTA4-HYDROLASE STUDY
Assay principle
The LTB4 kit is a competitive immunoassay for the measurement of LTB4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTB4 and XL665 coupled LTB4. LTB4 in the sample competes with XL665-labelled LTB4 for binding to anti-LTB4 Eu Cryptate.
Assay Protocol
The LTB4 assay features a streamlined protocol with only 1 incubation step after sample and LTB4 detection reagents dispensing.
This protocol requires a single two-hour incubation period at RT.
Key features
| LTB4 | |
|---|---|
| Incubation | 1h at RT |
| Assay range | 0.15 166.67 ng/mL |
| EC50 | 1.12 ng/mL |
| Detection limit | 0.15 ng/mL |
| Specificity | Low cross reactivity with 6-trans 12 epi LTB4 |
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Product Insert LTB4 Kit / 64LB4PEG-64LB4PEH
64LB4PEG-64LB4PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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