Human CCL20 (MIP3 alpha) kit
No-wash kit to quantify released Human CCL20 (MIP3 alpha)
Leukotriene B4 kit HTRF®
The Leukotriene B4 kit is designed for the accurate quantification of LTB4.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
LTB4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. LTB4 is synthesized by leukocytes via the 5-lipoxygenase pathway and mediates different leukocyte functions such as lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTB4 kit enables the monitoring of LTB4 produced by cells and can be used to study the LTA4-hydrolase.
Benefits
- CELLS AND CELL SUPERNATANT FORMATS
- LTA4-HYDROLASE STUDY
Assay principle
The LTB4 kit is a competitive immunoassay for the measurement of LTB4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTB4 and XL665 coupled LTB4. LTB4 in the sample competes with XL665-labelled LTB4 for binding to anti-LTB4 Eu Cryptate.
Assay Protocol
The LTB4 assay features a streamlined protocol with only 1 incubation step after sample and LTB4 detection reagents dispensing.
This protocol requires a single two-hour incubation period at RT.
Key features
| LTB4 | |
|---|---|
| Incubation | 1h at RT |
| Assay range | 0.15 166.67 ng/mL |
| EC50 | 1.12 ng/mL |
| Detection limit | 0.15 ng/mL |
| Specificity | Low cross reactivity with 6-trans 12 epi LTB4 |
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Product Insert LTB4 Kit / 64LB4PEG-64LB4PEH
64LB4PEG-64LB4PEH - Product Insert
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEG
64LB4PEG - Safety Data Sheet
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEH
64LB4PEH - Safety Data Sheet
Safety Data Sheet (DEU) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ELL) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ITA) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (SPA) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) LTB4 Kit / 64LB4PEJ
64LB4PEJ - Safety Data Sheet
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