Leukotriene C4 kit

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
See more
  • Ready-to-use
  • Highly specific
  • Faster and more convenient than ELISA
The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
-
In stock

Overview

LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.

Assay principle

The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.

Assay Protocol

The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

Key features

LTC4
Incubation 1h at RT
Assay range 0.1 – 5000 ng/mL
EC50 2.7 ng/mL
Detection limit 0.1 ng/mL
Specificity No cross reactivity with LTA4, LTB4
HTRF assays for Oncology and Inflammation

Signaling in the immune syqtem - Brochures

Product Insert LTC4 Kit / 64LC4PEG-64LC4PEH

64LC4PEG-64LC4PEH - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

tab6