Leukotriene C4 kit HTRF®

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Leukotriene C4 kit is designed for the accurate quantification of LTC4.


LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.



Assay principle

The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.
LTB4 kit assay principle

Assay Protocol

The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
LTB4 kit assay protocol

Key features

Incubation 1h at RT
Assay range 0.1 – 5000 ng/mL
EC50 2.7 ng/mL
Detection limit 0.1 ng/mL
Specificity No cross reactivity with LTA4, LTB4
LTC4 standard curve

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Plate Reader Requirement

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