Compounds disruption of Hsp90-Cdc37 interaction easily assessed in-vitro
MAb Anti 6HIS-XL665 HTRF®
- High affinity
MAb Anti-6HIS-XL665 is an IgG2a raised against a polyhistidine tagged fusion protein labeled with XL665. It recognizes synthetic polyhistidine or polyhistidine-tagged fusion protein.
This reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, protein/RNA, protein/carbohydrate, protein/small molecule, receptor/ligand.
HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.
- LARGE COMPLEXES DETECTION
- BATCH-TO-BATCH REPRODUCIBILITY
- LOW TO HIGH KD DETECTION
|Motif/Species||Antibody||Active Moiety/5,000 tests||Species and Subtype||Specificity|
|6HIS||MAb HIS-1||10-20 µg||Mouse IgG2a||HexaHistidine peptide|
In collaboration with Bristol Myers Squibb - Scientific Presentations
In collaboration with Scripps - Scientific Presentations
In collaboration with Sanford/Burnham - Scientific Presentations
Benefits and considerations of HTRF - Scientific Presentations
In collaboration with Lead Discovery Center GmbH - Scientific Presentations
In collaboration with MRC - Scientific Presentations
Benefit from unlimited flexibility for your assay development - Flyers
In collaboration with Boehringer Ingelheim - Posters
Every interaction is within range - Technical Notes
61HISXLF-61HISXLA-61HISXLB - Product Insert
Easy pharmacological characterization of PPI modulators. - Technical Notes
Deciphering low- and high affinity interactions - Application Notes
Monitoring nuclear receptor binding with HTRF assays - Application Notes
Challenge large complexes with HTRF assays - Application Notes
Get the brochure about technology comparison. - Brochures
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Plate Reader Requirement
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Description of the basics of the technology and review applications
HTRF used for the foundation of a cellular Ser/Thr-kinase platform that ...