MAb Anti-phospho CREB-Eu cryptate

MAb Anti-phospho CREB-Eu cryptate enables the rapid detection of CREB phosphorylation on Ser133 in a biochemical kinase assay format.

No-wash
Ease-of-use
High affinity

MAb Anti-phospho CREB-Eu cryptate enables the rapid detection of CREB phosphorylation on Ser133 in a biochemical kinase assay format.

Cat. number selected :

Quantity

In stock

Overview

The Anti-phospho CREB-Eu cryptate is a labeled monoclonal Ab enabling the rapid detection of CREB only when phosphorylated at Ser133. It does not cross-react with the corresponding non-phosphorylated sequence and also detects the phosphorylated form of the CREB-related protein ATF-1. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Benefits

NO LIMITATION IN ATP CONCENTRATIONS
ANY KIND OF SUBSTRATES
PHOSHO-CREB (SER133) SPECIFIC

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho CREB-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

tab6

Product Insert

Product Insert A-P CREB-Eu / 61P13KAE-61P13KAZ-61P13KAY
PDF (0.42 MB)

Latest news

8/14/18 Research Highlights Life Science

HTRF and its applications for kinase assays in dru...

A method for determining enzyme kinetic parameters (Km, Kcat) using ...

MAb Anti-phospho CREB-Eu cryptate

Overview

Benefits

tab6

Product Insert

Product Insert A-P CREB-Eu / 61P13KAE-61P13KAZ-61P13KAY

IP-One Gq kit

Phospho-AMPK (Thr172) cellular kit

Total NFkB cellular kit

Europe

America

Asia