HTRF KinEASE-STK S3 kit
A HTRF KinEASE kit to study serine/threonine kinases
MAb Anti-phospho CREB-Eu cryptate
MAb Anti-phospho CREB-Eu cryptate enables the rapid detection of CREB phosphorylation on Ser133 in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho CREB-Eu cryptate is a labeled monoclonal Ab enabling the rapid detection of CREB only when phosphorylated at Ser133. It does not cross-react with the corresponding non-phosphorylated sequence and also detects the phosphorylated form of the CREB-related protein ATF-1. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-CREB (SER133) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho CREB-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P CREB-Eu / 61P13KAE-61P13KAZ-61P13KAY
61P13KAE-61P13KAZ-61P13KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P CREB-Eu / 61P13KAE
61P13KAE - Safety Data Sheet
Safety Data Sheet A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P CREB-Eu / 61P13KAY
61P13KAY - Safety Data Sheet
Safety Data Sheet A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P CREB-Eu / 61P13KAZ
61P13KAZ - Safety Data Sheet
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