A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phosphoSAP/JNK-Eu cryptate HTRF®
MAb Anti-phospho SAP/JNK-Eu cryptate enables the rapid detection of SAP/JNK phosphorylation on Thr183 &Tyr184 15 in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho SAP/JNK-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of SAP/JNK only when phosphorylated at Thr183 & Tyr185. It does not cross-react with phosphorylated forms of p44/42 or p38 MAPK. It does not detect the non-phosphorylated SAP/JNK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-SAP/JNK (THR183/TYR185) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phosphoSAP/JNK-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P SAP/JNK-Eu / 61P09KAE-61P09KAZ-61P09KAY
61P09KAE-61P09KAZ-61P09KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P SAP/JNK-Eu / 61P09KAE
61P09KAE - Safety Data Sheet
Batch Information A-P SAP/JNK-Eu / 20230710
61P09KAE Batch 01 - Batch Information
Batch Information A-P SAP/JNK-Eu / 20231124
61P09KAE Batch 03A - Batch Information
Safety Data Sheet A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P SAP/JNK-Eu / 61P09KAY
61P09KAY - Safety Data Sheet
Safety Data Sheet A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P SAP/JNK-Eu / 61P09KAZ
61P09KAZ - Safety Data Sheet
Batch Information A-P SAP/JNK-Eu / 20231124
61P09KAZ Batch 03 - Batch Information
Plate Reader Requirement
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