Phospho-AKT3 (Ser473) cellular kit
Simple, all-in-one kit for robust detection of Phoshpo-ATK & Total ATK
MAP-Tau Kit HTRF®
The MAP-Tau Kit is designed for the accurate quantification of total Tau in cell lysates.
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Low sample consumption
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Highly specific
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Faster and more convenient than ELISA
Overview
MAP-Tau (microtubule-associated protein Tau) are soluble proteins required to stabilize microtubules through tubulin association. Deregulation of MAP-Tau expression and subsequent phosphorylation have been correlated to the onset of pathologies linked to the nervous system such as Alzheimer's and Parkinson's. The MAP-Tau assay enables the quantification of microtubule-associated protein Tau in cell lysates.
Benefits
- MAP-TAU STUDYIN ALZHEIMER'S DISEASE
- NORMALIZATION READOUT
Assay principle
MAP-Tau is measured using a sandwich immunoassay involving two specific anti-MAP-Tau antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the MAP-Tau present in the sample.
Assay Protocol
Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human MAP-Tau. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Product Insert MAP-Tau Kit / 63ADK008PEG-63ADK008PEH
63ADK008PEG-63ADK008PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Molecular basis of neuroinflammation and neurodegeneration diseases
The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides
Neurodegeneration and its main related diseases
Discover this infographic design on neurodegenerative diseases - Infographics
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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