MAP-Tau Kit
The MAP-Tau Kit is designed for the accurate quantification of total Tau in cell lysates.
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Low sample consumption
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Highly specific
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Faster and more convenient than ELISA
Overview
MAP-Tau (microtubule-associated protein Tau) are soluble proteins required to stabilize microtubules through tubulin association. Deregulation of MAP-Tau expression and subsequent phosphorylation have been correlated to the onset of pathologies linked to the nervous system such as Alzheimer's and Parkinson's. The MAP-Tau assay enables the quantification of microtubule-associated protein Tau in cell lysates.
Assay principle
MAP-Tau is measured using a sandwich immunoassay involving two specific anti-MAP-Tau antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the MAP-Tau present in the sample.
Assay Protocol
Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human MAP-Tau. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Cell-based assays for neurosciences
HTRF solutions - Flyers
Product Insert MAP-Tau Kit / 63ADK008PEG-63ADK008PEH
63ADK008PEG-63ADK008PEH - Product Insert
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides