Human CXCL1 (GRO alpha) kit
No-wash kit to quantify released Human CXCL1 (GRO alpha)
The HTRF mouse CCL2 (MCP1) kit is designed for the quantification of mouse CCL2 release in cell supernatant.
Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages, and dendritic cells to attract monocytes, basophils, and T cells. CCL2 is involved in pathologies like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes, and microglia, and is part of the neuro-inflammatory response observed in ischemia or Alzheimer's disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to diabetes.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse/rat CCL2/MCP1 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL2 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol|
|LOD &LOQ (in Diluent)||6 pg/mL & 13 pg/mL|
|Range||13 – 1,800 pg/mL|
|Time to result||ON at RT|
Intra assay (n=24)
|Sample||Mean [CCL2] (pg/mL)||CV|
Inter assay (n=4)
|Sample||[CCL2] (pg/mL)||Mean (delta ratio)||CV|
3T3-L1 cells* were plated in a 12 well plate at 30,000 cells per well under 1ml. Cells were grown until confluency, and differentiated onto adipocyte (~700,000 cells per well). Differentiated cells were then stimulated for 24h with IL1ß and TNFa, both used at 10ng/ml. Supernatants were diluted 100 times. 16 µL
of diluted supernatants were transferred into a 384 well (SV) white detection plate to be analyzed by the Mouse CCL2 Assay Kit.
*Samples were kindly provided by J.F. Tanti’s research team: Cellular and Molecular Physiopathology of Obesity, Inserm U1065, Nice, FRANCE.
In collaboration with CNRS - Scientific Presentations
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
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