Mouse Fc kit HTRF®

The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.


Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Mouse Fc kit enables a rapid quantification of mouse IgG, and any proteins containing a mouse Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.



Assay principle

The mouse Fc kit is a competitive immunoassay for the measurement of mIgGs or any mFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to mFc and XL665 coupled mIgGs. mFc contained in the sample competes with XL665-labelled mouse IgG for binding to anti-mouse Fc Eu Cryptate.
Mouse Fc kit assay principle

Assay Protocol

The mouse Fc assay features a streamlined protocol with only 1 incubation step after sample and Mouse Fc detection reagents dispensing. This protocol requires a single two-hour incubation period.
Mouse Fc kit assay protocol

Key features

Mouse Fc
Detection limit: 6.8 ng/mL
Dynamic range: 0.8 – 400,000 ng/mL
Incubation time: 2h at RT
EC50: 138 ng/mL
mouse Fc standard curve

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Plate Reader Requirement

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