Mouse IFN gamma kit HTRF®

Name: Mouse IFN gamma kit
Brand: Cisbio
SKU: 40458
Availability: InStock

Associated products

The HTRF Mouse IFN gamma kit is designed for the quantification of mouse IFN gamma released in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF Mouse IFN gamma kit is designed for the quantification of mouse IFN gamma released in cell supernatant.

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Overview

IFN gamma is a pro-inflammatory cytokine secreted by T and NK cells in response to viral infection and other inflammatory conditions. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and the development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in the treatment of osteoporosis and it is of great interest in immuno-oncology, where it improves survival in patients with bladder or ovarian carcinoma.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse IFN-γ AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536 WELLS
T CELL FUNCTIONAL READOUT

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio has also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IFN gamma analysis.

Technical specifications of mouse IFN gamma kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	23 pg/mL &34 pg/mL
Range	34 3,000 pg/mL
Time to result	2h at RT
Species	Mouse only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IFNg] (pg/mL)	CV
1	80	11%
2	1375	2%
3	2750	2%
	Mean CV	5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IFNg] (pg/mL)	Mean (delta R)	CV
1	94	213	5%
2	375	868	10%
3	1500	3502	13%
		Mean CV	9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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