Human CCL20 (MIP3 alpha) kit
No-wash kit to quantify released Human CCL20 (MIP3 alpha)
Mouse IL6 kit HTRF®
The HTRF mouse IL6 kit is designed for the quantification of mouse IL6 release in cell supernatant.
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No-wash
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Low sample consumption
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Under 2h bench time
Overview
IL6 is a pro-inflammatory cytokine involved in acute-phase reaction, inflammation, and cancer progression. Secreted by T cells, macrophages, and fibroblasts, it induces B and T cell proliferation. It is being studied in a wide variety of research areas including diabetes, Alzheimer's disease, depression, and several cancers. Along with TGF beta, IL6 is the main promoter of T cell differentiation into TH17, a new component of immuno-oncology.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse IL-6 AlphaLISA kit and the Human IL-6 AlphaLISA biotin-free kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
Benefits
- SCALABLE FROM 96 TO 1536WELLS
- B CELL ACTIVATION READOUT
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL6 analysis.
Technical specifications of mouse IL6 kit
| Sample size | 16 µL |
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 6 pg/mL &19 pg/mL |
| Range | 19 7,000 pg/mL |
| Time to result | 1h at RT |
| Calibration | NIBSC (93/730) value (IU/mL) = 0,7 x HTRF mIL6 value (pg/mL) |
| Species | Mouse only |
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL6] (pg/mL) | CV |
|---|---|---|
| 1 | 40 | 12% |
| 2 | 1400 | 7% |
| 3 | 2800 | 11% |
| Mean CV | 10% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL6] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 72 | 503 | 6% |
| 2 | 448 | 3188 | 10% |
| 3 | 2800 | 18091 | 10% |
| Mean CV | 9% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF and Alpha solutions for NASH - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the Immune System - Brochures
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
Webinar: NASH - Fibrosis Research and Drug Discovery
Featuring a panel of experts - Videos
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert IL6 (m) Kit / 62MIL06PEG-62MIL06PEH
62MIL06PEG-62MIL06PEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of Life Sciences Cytokine Assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Protocol for Th17 differentiation from CD4 mouse T-lymphocyte
A step by step protocol for Th17 cells differentiation from naive CD4 mouse cells - Technical Notes
An innate and adaptive immunity recap
Insight into the diversity of cells & signaling pathways - Guides
Three drug discovery approaches for Multiple Sclerosis
Power up your multiple sclerosis research projects with this technical note - Application Notes
Neutralizing antibodies in Autoimmunity
Power up your autoimmunity research projects with this application note - Application Notes
Assays for autoimmunity research
Advance your research on autoimmune diseases - Flyers
Assays for neurosciences research
Advance your research on neurodegenerative diseases - Flyers
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Safety Data Sheet (DEU) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL6 (m) Kit / 62MIL06PEG
62MIL06PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL6 (m) Kit / 62MIL06PEH
62MIL06PEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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