Mouse TNF alpha kit HTRF®

Name: Mouse TNF alpha kit
Brand: Cisbio
SKU: 40468
Availability: InStock

Associated products

The HTRF mouse TNF alpha kit is designed for the quantification of mouse TNF alpha release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

Download the protocols

The HTRF mouse TNF alpha kit is designed for the quantification of mouse TNF alpha release in cell supernatant.

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Overview

TNF alpha is a chemokine mainly produced by macrophages, T cells, and neutrophils. TNF alpha acts as a chemoattractant for neutrophils, and promotes infiltration and activation at inflammation sites. TNF alpha is involved in several cancer types for its ability to promote angiogenesis and cell proliferation, as well as to inhibit apoptosis.

Benefits

SCALABLE FROM 96 TO 1536WELLS
PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your TNF alpha analysis.

Technical specifications of mouse TNF alpha kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	3 pg/mL &20 pg/mL
Range	20 6,000 pg/mL
Time to result	1h at RT
Species	Mouse only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [TNFa] (pg/mL)	CV
1	57	7%
2	2570	2%
3	5256	2%
	Mean CV

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[TNFa] (pg/mL)	Mean (delta R)	CV
1	93	457	2%
2	493	2182	3%
3	2609	8729	3%
		Mean CV	3%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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