Opioid Delta, Mu and KappaR fluorescent probe
Tag-lite Opioid Delta & Kappa receptors fluorescent probe
Mu opioid R expressing cells labeled with Terbium HTRF®
Tag-lite cells transiently expressing the Mu opioid receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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Fully functional cell line
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Transient expression
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Cellular binding assay
Overview
One of the major subtypes of opioid receptors, Mu Opioid (or MOP) is predominantly expressed in the brain. The Mu opioid receptor is highly involved in the nociception and stress systems. MOP ligands are most commonly used to treat acute and chronic pain, cough, digestive disorders, and psychiatric disorders characterized by reward dysfunction.
Cells expressing the MOP receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Benefits
- PRE-LABELED WITH TERBIUM
- FROZEN CELLS, READY TO BE USED
- CELLS/LIGAND VALIDATED FOR BINDING
Assay principle
Running your Mu opioid receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand (L0005RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Kd and Ki validation
Examples of data obtained using Mu opioid labeled cells and their matching fluorescent ligand (L0005RED). Naltrindole was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Challenge the limits of binding kinetics studies - Application Notes
HTRF and Tag-lite technologies in multidimensional cell-based assays for GPCR investigations
16 GPCR challenges, 1 solution - Posters
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
Product Insert m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Product Insert
Product Insert m-opioid-R LabCell / CC1TT1MOP
CC1TT1MOP - Product Insert
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet (DEU) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (ELL) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (FRA-FR) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (ITA) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (SPA) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (ENG-GB) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Safety Data Sheet (ENG-US) m-opioid-R LabCell / C1TT1MOP
C1TT1MOP - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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