-->
Search

PAb Anti Mouse IgG-Tb cryptate HTRF®

  • Photography of the PAb Anti Mouse IgG-Tb cryptate vial
  • Photography of the PAb Anti Mouse IgG-Tb cryptate vial
  • A fun video introducing you to PPi assays with HTRF
Tb cryptate-labeled anti-mouse IgG antibody for capturing anti-mouse IgG-tagged proteins in protein/protein interaction assays.
See more
  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • High affinity High affinity
Download the protocols
Tb cryptate-labeled anti-mouse IgG antibody for capturing anti-mouse IgG-tagged proteins in protein/protein interaction assays.
-
In stock

Prices may differ between countries. Request a quote to receive exact pricing.

Overview

IgGs from Rabbit anti-Mouse immunoserum were immunopurified and labeled with Tb. PAb Anti Mouse IgG-Tb cryptate may be used to detect the binding of unlabeled mouse specific antibody on a target molecule or on Ig fusion proteins.

HTRF can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, protein/RNA, protein/carbohydrate, protein/small molecule, receptor/ligand.

HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.

Benefits

  • LARGE COMPLEXES DETECTION
  • BATCH-TO-BATCH REPRODUCIBILITY
  • LOW TO HIGH KD DETECTION

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners.

In the example shown here: PAb Anti Mouse IgG-Tb cryptate binds to the Mouse Fc fused tagged partner A while partner B* binds to a specific Ab labeled with an HTRF acceptor.

*partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.

assay principle of detection assay using HTRF PAb Anti Mouse IgG-Tb cryptate

Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Mouse Fc fused-tagged partner A and a non-tagged partner B*.

Dispense the 2 partners (10 µL), incubate, add PAb Anti Mouse IgG-Tb cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate and read.

*partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with acceptor for the detection.

assay protocol of detection assay using HTRF PAb Anti Mouse IgG-Tb cryptate

How do the number of tests relate to active moiety?

The average conjugate quantity per well reflects overall biological material content. Using the active moiety amount is generally preferred to the quantity of total conjugate. For Cryptate and d2 conjugates, the total conjugate amount equals that of the active moiety, since the molecular weight of the label is negligible. This is not the case for XL665 labeled entities for which the quantity of total conjugate will vary depending on the final molar ratio of the XL665 conjugate, however, the amount of active moiety, provided by Cisbio, is constant and based on the number of tests ordered.
description of the active moiety in an HTRF conjugate

Recommended quantities of Cryptate and XL665 conjugates

Cryptate conjugates must not be excessive in order to prevent reader saturation and an unacceptable level of background. In most cases, a cryptate concentration of 1 to 5nM is appropriate, and will generate 20,000 to 80,000 cps at 620 nm depending on the HTRF compatible reader used. The XL665 conjugate must match its assay counterpart as closely as possible in order for the maximum number of biomolecules to be tagged with the XL665 acceptor. Thus, to detect a tagged molecule at an assay concentration of 20nM, the concentration of anti-Tag-XL665 should be equimolar or higher.

Product Insert Anti (m) IgG-Tb / 61PAMTAF-61PAMTAA-61PAMTAB

61PAMTAF-61PAMTAA-61PAMTAB - Product Insert

HTRF PPI your dream assay served on a plate

Sandwiches aren't just for eating - Infographics

Best practices for pharmacological characterization of PPI inhibitors

Easy pharmacological characterization of PPI modulators. - Technical Notes

HTRF assays handle low- to high affinity protein-protein interactions

Deciphering low- and high affinity interactions - Application Notes

Nuclear receptor ligand identification with HTRF

Monitoring nuclear receptor binding with HTRF assays - Application Notes

HTRF addresses large protein-protein interaction complexes

Challenge large complexes with HTRF assays - Application Notes

A brief history of Protein-Protein Interactions

How well do you know PPI? - Infographics

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Virology research solutions using HTRF Protein-Protein Interaction assays

See how peer researchers challenge the viral life cycle with PPI assays - Application Notes

Solutions for CAR-T research

Advance your CAR-T cell research - Flyers

Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAA

61PAMTAA - Safety Data Sheet

Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAB

61PAMTAB - Safety Data Sheet

Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAF

61PAMTAF - Safety Data Sheet

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

Let's find your reader

Guides

The ultimate guide to PPI assays with HTRF

Technical Notes

Guidelines for optimizing protein-protein interaction assays...

Application Notes

HTRF assays handle low- to high affinity protein-protein int...