PAb Anti Mouse IgG-Tb cryptate HTRF®
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No-wash
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Ease-of-use
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High affinity
Overview
IgGs from Rabbit anti-Mouse immunoserum were immunopurified and labeled with Tb. PAb Anti Mouse IgG-Tb cryptate may be used to detect the binding of unlabeled mouse specific antibody on a target molecule or on Ig fusion proteins.
HTRF can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, protein/RNA, protein/carbohydrate, protein/small molecule, receptor/ligand.
HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.
Benefits
- LARGE COMPLEXES DETECTION
- BATCH-TO-BATCH REPRODUCIBILITY
- LOW TO HIGH KD DETECTION
Assay principle
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners.
In the example shown here: PAb Anti Mouse IgG-Tb cryptate binds to the Mouse Fc fused tagged partner A while partner B* binds to a specific Ab labeled with an HTRF acceptor.
*partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Mouse Fc fused-tagged partner A and a non-tagged partner B*.
Dispense the 2 partners (10 µL), incubate, add PAb Anti Mouse IgG-Tb cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate and read.
*partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with acceptor for the detection.
How do the number of tests relate to active moiety?
Recommended quantities of Cryptate and XL665 conjugates
Product Insert Anti (m) IgG-Tb / 61PAMTAF-61PAMTAA-61PAMTAB
61PAMTAF-61PAMTAA-61PAMTAB - Product Insert
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Advance your CAR-T cell research - Flyers
Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAA
61PAMTAA - Safety Data Sheet
Batch Information Anti (m) IgG-Tb / 20221109
61PAMTAA Batch 03B - Batch Information
Batch Information Anti (m) IgG-Tb / 20221109
61PAMTAA Batch 03C - Batch Information
Batch Information Anti (m) IgG-Tb / 20241109
61PAMTAA Batch 03B - Batch Information
Batch Information Anti (m) IgG-Tb / 20241109
61PAMTAA Batch 03C - Batch Information
Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAB
61PAMTAB - Safety Data Sheet
Batch Information Anti (m) IgG-Tb / 20221109
61PAMTAB Batch 03B - Batch Information
Batch Information Anti (m) IgG-Tb / 20241109
61PAMTAB Batch 03B - Batch Information
Safety Data Sheet (DEU) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (ELL) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (ITA) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (SPA) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti (m) IgG-Tb / 61PAMTAF
61PAMTAF - Safety Data Sheet
Batch Information Anti (m) IgG-Tb / 20221109
61PAMTAF Batch 03B - Batch Information
Batch Information Anti (m) IgG-Tb / 20241109
61PAMTAF Batch 03B - Batch Information
Plate Reader Requirement
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