PolyGT-Biotin substrate
PolyGT;Biotin is a substrate for tyrosine;specific protein kinases
PAb Anti-phospho PKA substrate-Eu cryptate HTRF®
PAb Anti-phospho PKA substrate-Eu cryptate enables the detection of phosphorylation of peptides or proteins on Ser/Thr in a biochemical kinase assay format.
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No-wash
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Ease-of-use
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High affinity
Overview
The Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrates can be used, from small synthetic peptides, to natural substrates for a high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
Benefits
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(SER/THR) SPECIFIC
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assay have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho PKA substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
Product Insert A-P PKA-Eu / 61P01KAE-61P01KAZ-61P01KAY
61P01KAE-61P01KAZ-61P01KAY - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (DEU) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (ELL) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (ITA) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (SPA) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P PKA-Eu / 61P01KAE
61P01KAE - Safety Data Sheet
Batch Information A-P PKA-Eu / 20230305
61P01KAE Batch 04A - Batch Information
Batch Information A-P PKA-Eu / 20230901
61P01KAE Batch 03 - Batch Information
Safety Data Sheet A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (DEU) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (ELL) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (ITA) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (SPA) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P PKA-Eu / 61P01KAY
61P01KAY - Safety Data Sheet
Safety Data Sheet A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (DEU) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (ELL) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (FRA-FR) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (ITA) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (SPA) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (ENG-GB) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Safety Data Sheet (ENG-US) A-P PKA-Eu / 61P01KAZ
61P01KAZ - Safety Data Sheet
Batch Information A-P PKA-Eu / 20230305
61P01KAZ Batch 04 - Batch Information
Batch Information A-P PKA-Eu / 20230901
61P01KAZ Batch 03 - Batch Information
Plate Reader Requirement
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