Phospho-alpha Synuclein (Ser129) cellular kit HTRF®

This HTRF kit enables the cell-based detection of phosphorylated alpha-synuclein at Ser129 as a marker of neurodegenerative diseases.

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  • All inclusive kit All inclusive kit
  • High sensitivity High sensitivity
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
  • No-wash No-wash

This HTRF kit enables the cell-based detection of phosphorylated alpha-synuclein at Ser129 as a marker of neurodegenerative diseases.



The HTRF Phospho-alpha Synuclein (Ser129) cell-based assay kit is ideal for quantifying endogenous phospho-alpha Synuclein phosphorylated on Serine 129.

The alpha Synuclein protein is involved in the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson’s disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA).



Phospho-Synuclein (Ser129) assay principle

The Phospho-alpha Synuclein (Ser129) assay measures Synuclein when phosphorylated at Ser129. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer.

The Phospho-Synuclein (Ser129) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. The first antibody was selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. Protein phosphorylation enables an immune-complex formation involving both the labeled antibodies, and which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of phosphorylated protein present in the sample, and provides a means of assessing the protein’s phosphorylation state under a no-wash assay format.

Phospho-TBK1 (Ser172) assay principle

Phospho-alpha Synuclein (Ser129) 2-plate assay protocol

The 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before adding Phospho-alpha Synuclein (Ser129) HTRF detection reagents.

This protocol enables the cells' viability and confluence to be monitored.

Phospho-TBK1 (Ser172) 2-plate assay protocol

Phospho-alpha Synuclein (Ser129) 1-plate assay protocol

Detection of Phospho-alpha Synuclein (Ser129) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required.

This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.

Phospho-TBK1 (Ser172) 1-plate assay protocol

Phosphorylation of alpha Synuclein on Serine 129 in Human samples

HEK293 cells were transfected with a plasmid encoding alpha-Synuclein prior to seeding treatment with non phosphorylated fibrils. After cell lysis, different dilutions of lysate were analyzed by HTRF.

Phosphorylation of alpha synuclein was detected in human brain homogenates from patients with Lewy Body Diseases.

HEK293 cells transfected with alpha-Synuclein treated with fibril
Validation of phospho alpha synuclein (Ser129) kit in human brain samples

Phosphorylation of alpha Synuclein on Serine 129 in Mouse samples

The alpha Synuclein phosphorylation was detected on primary neurons obtained from WT mouse .

Inducible recombinant mouse N2A cells treated with non phosphorylated fibrils displayed high phosphorylation levels of alpha Synuclein.

Validation of phospho alpha synuclein (Ser129) kit in mouse neuron lysates
Validation of phospho alpha synuclein (Ser129) kit in mouse samples

Parkinson's disease pathway and a-synuclein role

Alpha-Synuclein is a prominent component of intracellular fibrillary aggregates in the brains of patients suffering from synucleinopathies. This protein represents a key molecular hallmark for Parkinson’s disease, dementia with Lewy bodies, or multiple system atrophy. The extensive phosphorylation of alpha synuclein on Ser129 is also associated with a pathological event.

Parkinson's disease pathway

Analysis of the effect of aggregated beta-Amyloid on cellular signaling pathways critical for memory in Alzheimer's disease

Changes in CREB and ERK phosphorylation levels after beta-amyloid treatment - Application Notes

Detection of human tau protein aggregation

For researcher working on PD or AD - Application Notes

Lysis buffer compatibility

Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers

Species compatibility

Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers

HTRF Alpha-tubulin Housekeeping kit

Properly interpret your compound effect - Application Notes

Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

Key guidelines to successful cell signaling experiments

Mastering the art of cell signaling assays optimization - Guides

Exploring LRRK2 phosphorylation with HTRF® assays

LRRK2 kit, a game changer in neuroscience - Posters

Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences

Insider Tips for successful sample treatment - Technical Notes

Exploring Synuclein phosphorylation and aggegation with HTRF assays

Alpha Synuclein kits, perfect tools to study synucleinopathy - Posters

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Best practices for analyzing tumor xenografts with HTRF phospho assays

Protocol for tumor xenograft analysis with HTRF - Technical Notes

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

Tumor xenograft analysis: HTRF versus Western blot - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

How to run a cell based phospho HTRF assay

What to expect at the bench - Videos

Molecular basis of neuroinflammation and neurodegeneration diseases

The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

Neurodegeneration and its main related diseases

Discover this infographic design on neurodegenerative diseases - Infographics

Assays for neurosciences research

Advance your research on neurodegenerative diseases - Flyers

Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection

Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes

Assessment of drug efficacy and toxicity by combining innovative technologies

Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes

Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)

Learn how to reduce time and sample consumption - Application Notes

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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