Phospho and Total protein blocking reagent HTRF®
Overview
Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.
Benefits
- SPECIFICITY
- PRECISION
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (DEU) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (ELL) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (FRA-FR) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (ITA) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (SPA) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (ENG-GB) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Safety Data Sheet (ENG-US) P+T protein BlockRgt / 64KB1AAC
64KB1AAC - Safety Data Sheet
Batch Information P+T protein BlockRgt / 20231217
64KB1AAC Batch 25A - Batch Information
Batch Information P+T protein BlockRgt / 20240919
64KB1AAC Batch 23D - Batch Information
Batch Information P+T protein BlockRgt / 20240919
64KB1AAC Batch 23F - Batch Information
Batch Information P+T protein BlockRgt / 20250224
64KB1AAC Batch 26A - Batch Information
Batch Information P+T protein BlockRgt / 20240214
64KB1AAC Batch 21A - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23A - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23B - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23C - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23D - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23E - Batch Information
Batch Information P+T protein BlockRgt / 20250919
64KB1AAC Batch 23F - Batch Information
Batch Information P+T protein BlockRgt / 20250703
64KB1AAC Batch 22A - Batch Information
Batch Information P+T protein BlockRgt / 20250703
64KB1AAC Batch 22B - Batch Information
Batch Information P+T protein BlockRgt / 20250703
64KB1AAC Batch 22C - Batch Information
Batch Information P+T protein BlockRgt / 20250703
64KB1AAC Batch 22D - Batch Information
Batch Information P+T protein BlockRgt / 20260403
64KB1AAC Batch 28A - Batch Information
Safety Data Sheet P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (DEU) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (ELL) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (FRA-FR) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (ITA) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (SPA) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (ENG-GB) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Safety Data Sheet (ENG-US) P+T protein BlockRgt / 64KB1AAD
64KB1AAD - Safety Data Sheet
Batch Information P+T protein BlockRgt / 20231217
64KB1AAD Batch 25A - Batch Information
Batch Information P+T protein BlockRgt / 20250224
64KB1AAD Batch 26A - Batch Information
Batch Information P+T protein BlockRgt / 20250224
64KB1AAD Batch 26RA - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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