Total Phospholamban cellular kit
All-in-one kit for robust detection of Phospho-Phospholamban
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This cell-based assay enables the detection of Phosphorylated Phospholamban (PLN) at Threonine 17. The phospho assay along with the total PLN assay are ideal for monitoring the activation of CaMKII. The increase in intracellular Ca2+ causes the activation of CaMKII, which in turn phosphorylates PLN at the Thr17 residue. Dephosphorylated PLN inhibits SERCA2a, whereas phosphorylation of PLN at either the Ser16 site by PKA or the Thr17 site by CaMKII reverses this SERCA2a inhibition. The PLN phosphorylation state regulates the activity of this Ca2+ pump.
Phospholamban (PLN) plays a crucial role in heart failure through its control of cardiac sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA2a). This protein has a Ca2+ pump included in the SR membrane, and once activated, Ca2+ goes inside SR. Insufficient SERCA2a activity is a hallmark of heart failure. Dephosphorylated PLN inhibits SERCA2a, whereas phosphorylation of PLN at either the Ser16 site by PKA or the Thr17 site by CaMKII reverses the inhibition of SERCA2a. The PLN phosphorylation state regulates the activity of this Ca2+ pump. Relaxation is driven by dephosphorylation of PLN, and contraction by the phosphorylation status.
This small protein, is present in cardiac, smooth, and slow-twitch skeletal muscles. However, its regulatory effects have mainly been studied in cardiac muscle. The activation process is not well known, but 2 main pathways are described ending in 2 different phosphaorylations: on residue Serine 16, or on Threonine 17. Binding of a β-agonist to its receptor activates G protein, which enhances adenylate cyclase (AC) activity. AC catalyzes cAMP formation, which activates PKA. PKA phosphorylates the L-type Ca2+ channel, increasing the Ca2+ influx, and phosphorylates PP1 and the Ser16 residue of PLN. An increase in intracellular Ca2+ causes the activation of CaMKII. This autophosphorylation state is also controlled by PP1. CaMKII in turn phosphorylates PLN at the Thr17 residue.
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In collaboration with Sanofi - Scientific Presentations
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HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
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Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
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Unmatched ease of use, sensitivity and specificity assays - Videos
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Product Insert PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Product Insert
Product Insert PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
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Get the brochure about technology comparison. - Brochures
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3' video to set up your Phospho assay - Videos
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Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
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Safety Data Sheet (DEU-) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (ELL-) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (ITA-) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (SPA-) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) PLamban P-T17 Kit / 63ADK074PEG
63ADK074PEG - Safety Data Sheet
Safety Data Sheet (DEU-) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (ELL-) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (ITA-) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (SPA-) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) PLamban P-T17 Kit / 63ADK074PEH
63ADK074PEH - Safety Data Sheet
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