Phospho-SMAD1 (Ser463/465) cellular kit
- High sensitivity
- Faster and more convenient than ELISA
- Low sample consumption
This HTRF cell-based assay enables rapid, quantitative detection of SMAD1 phosphorylated at Serine 463/465. After phosphorylation and translocation to the nucleus, SMAD1 acts as a transcription factor in regulating the expression of genes involved in stem cell renewal, cell proliferation, apoptosis, migration, differentiation, and immune responses.
Mouse C2C12 cells were cultured to 80% confluency. After BMP-4 treatment, cells were lysed and soluble supernatants were collected via centrifugation. Serial dilutions of the cell lysate were performed and 16 µL of each dilution were transferred into a 384-well low volume white microplate before finally adding phospho kit reagents. A side by side comparison showed the HTRF Phospho assay is at least 8-fold more sensitive than the Western Blot, and shows optimal correlation.
BMP/SMAD signaling pathway plays a key role in embryogenesis, development, adult tissue homeostasis and immune responses. It is mediated by heterotetrameric complexes of BMPRI and BMPRI, that activate the intracellular signaling proteins SMAD1/5/8 by phosphorylating two C-terminal serine residues.
BMP-2/4 binding on BMPRI triggers the recruitment of BMPRII, which then transphosphorylates the intracellular domain of BMPRI.
BMPRI in turn phosphorylates SMAD1 at Ser463 and Ser465, enabling its oligomerization with the co-mediator (Co-SMAD) SMAD4. This complex translocates to the nucleus, where it functions as a transcription factor with coactivators and corepressors to regulate gene expression.
Inhibitory SMAD6 and SMAD7 are involved in feedback inhibition of the signaling pathway.
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
Physiologically relevant results fo fast flowing research - Flyers
Do all cell-based kinase assays perform similarly? - Posters
Simplify complex pathway dissection by combining the power of HTRF® cellular phospho-assays and the flexibility of the CyBi®-FeliX liquid handling system
PI3K/AKT/mTor translational control pathway - Posters
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
Increased flexibility of phospho-assays - Application Notes
HTRF® phospho-protein platform facilitates the dissection of signaling pathways involved in insulin resistance and metabolic disorders
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Insider Tips for successful sample treatment - Technical Notes
HTRF and WB compatible guidelines - Technical Notes
Mastering the art of cell signaling assays optimization - Guides
Detailed protocol and direct comparison with WB - Posters
Implement HTRF phospho-protein assays at every step of the drug discovery process, from in vitro to in vivo models
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Unmatched ease of use, sensitivity and specificity assays - Videos
In collaboration with Bayer - Scientific Presentations
63ADK062PEG-63ADK062PEH - Product Insert
Get the brochure about technology comparison. - Brochures
A solution for phospho-protein analysis in metabolic disorders - Posters
A fun video introducing you to phosphorylation assays with HTRF - Videos
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