Phospho-STAT3 (Tyr705) cellular kit
All-in-one kit for robust detection of Phospho-STAT3 (Ser423/425)
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This HTRF kit enables the cell-based quantitative detection of phosphorylated STAT5 at Tyr694, as a readout of the JAK/STAT pathway.
The Phospho-STAT5 (Tyr694) kit is designed to robustly and efficiently monitor STAT5 phosphorylation on Tyr694 , as a readout of JAK/STAT pathway activation. With an optimized and streamlined mix-and-read, no-wash protocol, the phospho-STAT5 contains all reagents you need for your research to run faster and more efficiently.
TF1 cells were plated in 25µl of cell culture medium treated for 30min with 5µl of increasing concentrations of the JAK inhibitor Lestaurtinib. After removal of cell culture medium, 10µl of 4X supplemented lysis buffer were added and incubated for 30 minutes prior to the addition of the HTRF detection reagents. 16µl of lysates were transferred in a 384sv white plate for analysis of STAT5 phosphorylation. The results clearly demonstrate, that the high sensitivity of the assay allows a 4-fold lower cell quantity to be used without a significant decrease of assay window.
20,000 TF1 cells were plated directly in a 384sv plate suspended in 8µl of cell culture medium treated for 30min with 4µl of increasing concentrations of the JAK inhibitor Lestaurtinib. After removal of cell culture medium, 4µl of 4X supplemented lysis buffer were added and incubated for 30 minutes prior to the addition of the HTRF detection reagents. STAT5 phosphorylation was then measured using the HTRF® phospho-STAT5 (Tyr694) assay.
100KC HeLa cells were plated in 25µl of cell culture medium treated for 2h with 5µl of increasing concentrations of the JAK inhibitor Lestaurtinib and then stimulated with 20 nM IFN for 10min. After removal of cell culture medium, 10µl of 4X supplemented lysis buffer were added and incubated for 30 minutes prior to the addition of the HTRF detection reagents. 16µl of lysates were transferred in a 384sv white plate for analysis of STAT5 phosphorylation.
Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
HTRF cellular phospho-protein assays
Physiologically relevant results fo fast flowing research - Flyers
HTRF solutions for each cell type - Flyers
Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers
PI3K/AKT/mTor translational control pathway - Posters
Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells
Analysis of a large panel of diverse biological samples and cellular models - Posters
From 2D, 3D cell cultures to xenografts: A smart HTRF platform to maximize anticancer drug discovery
One technology across all samples - Application Notes
HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
HTRF cell-based phospho-protein data normalization
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers
Increased flexibility of phospho-assays - Application Notes
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Best practices for analyzing brain samples with HTRF® phospho assays for neurosciences
Insider Tips for successful sample treatment - Technical Notes
HTRF Alpha-tubulin Housekeeping kit
Properly interpret your compound effect - Application Notes
Optimize your HTRF cell signaling assays on tissues
HTRF and WB compatible guidelines - Technical Notes
Key guidelines to successful cell signaling experiments
Mastering the art of cell signaling assays optimization - Guides
HTRF phospho-assays reveal subtle drug-induced effects
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Best practices for analyzing tumor xenografts with HTRF phospho assays
Protocol for tumor xenograft analysis with HTRF - Technical Notes
How to run a cell based phospho HTRF assay
What to expect at the bench - Videos
Unleash the potential of your phosphorylation research with HTRF
Unmatched ease of use, sensitivity and specificity assays - Videos
Cell-based kinase assays in HTS ? potential and limitations for primary and secondary screening
In collaboration with Bayer - Scientific Presentations
Product Insert STAT5 P-Y694 Kit / 64AT5PEG-64AT5PEH
64AT5PEG-64AT5PEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
HTRF® cell signaling platform combined with iCell® Hepatocytes
A solution for phospho-protein analysis in metabolic disorders - Posters
Unleash the potential of your phosphorylation research with HTRF
A fun video introducing you to phosphorylation assays with HTRF - Videos
How to run a cell based phospho HTRF assay
3' video to set up your Phospho assay - Videos
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Inhibition of FGFR Signaling Pathways Studied in Cancer Cell Lines Using HTRF
The knowledge and data necessary to understand the inhibition value of FGFR receptors involved in human cancers. HTRF technology enables the monitoring of the activity of the AZD4547 inhibitor on cell signal transduction in 3 cancer cell lines. - Application Notes
Advance your research on Fibrosis
Kits and reagents for Fibrosis research - Flyers
Guidelines for Cell Culture and Lysis in Different Formats Prior to HTRF Detection
Seeding and lysing recommendations for a number of cell culture vessels. - Technical Notes
Assessment of drug efficacy and toxicity by combining innovative technologies
Combination of AlphaLISA®, HTRF®, or AlphaLISA® SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay - Application Notes
Methodological Aspects of Homogeneous Time-Resolved Fluorescence (HTRF)
Learn how to reduce time and sample consumption - Application Notes
Safety Data Sheet STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (DEU) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (ELL) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (ITA) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (SPA) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) STAT5 P-Y694 Kit / 64AT5PEG
64AT5PEG - Safety Data Sheet
Safety Data Sheet (DEU) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (ELL) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (ITA) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (SPA) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) STAT5 P-Y694 Kit / 64AT5PEH
64AT5PEH - Safety Data Sheet
Safety Data Sheet (DEU) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (ELL) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (FRA-FR) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (ITA) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (SPA) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (ENG-GB) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
Safety Data Sheet (ENG-US) STAT5 P-Y694 Kit / 64AT5PEY
64AT5PEY - Safety Data Sheet
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