Cynomolgus TNF alpha kit
No-wash kit to quantify released Cynomolgus TNF alpha
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Prostaglandin E2 (PGE2) is produced in multiple cell types from PGH2, a primary product of the arachidonic acid metabolism, via the prostaglandin synthase. PGE2 displays several biological functions, such as vasodilatation and smooth muscle relaxation, and is involved in pro- and anti-inflammation pathways. PGE2 production is a commonly used method for the detection of COX-1 and COX-2 modulation. The PGE2 kit is a highly sensitive method for quantifying PGE2, either in cell supernatant or directly in whole cells.
Detection limit | 10 pg/mL |
Dynamic range | 8 to 1000 pg/mL |
EC50 | 250 pg/mL |
S/B | 31 |
Z' | 0.9 for 20 µl assay volumes |
One benefit of the HTRF PGE2 assay is its ability to quantify PGE2 in the presence of cells without sacrificing assay performance. The results comparing direct cell-based versus supernatant transfer did not differ significantly. Monocytes were stimulated to produce PGE2 in the presence or absence of indomethacin, a known inhibitor of PGE2 production. The IC50 of indomethacin determined using HTRF technology (1.0 ± 0.4 nM) was in agreement with previously published data.
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Product Insert PGE2 Kit / 62P2APEG-62P2APEH
62P2APEG-62P2APEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
HTRF and AlphaLISA assays bring clarity to extracellular matrix related fibrosis development
Fibrosis study investigating the role of the extracellular matrix in fibrosis disorder - Application Notes
Safety Data Sheet (DEU) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (ELL) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (ITA) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (SPA) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (ENG-US) PGE2 Kit / 62P2APEG
62P2APEG - Safety Data Sheet
Safety Data Sheet (DEU) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (ELL) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (ITA) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (SPA) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (ENG-US) PGE2 Kit / 62P2APEH
62P2APEH - Safety Data Sheet
Safety Data Sheet (DEU) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (ELL) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (ITA) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (SPA) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) PGE2 Kit / 62P2APEJ
62P2APEJ - Safety Data Sheet
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