Mouse Fc kit
Mouse Fc quantification for bioprocess applications
Rabbit Fc kit HTRF®
The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Rabbit Fc kit enables a rapid quantification of rabbit IgG and any proteins containing a rabbit Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
Benefits
- LOW SAMPLE CONSUMPTION
- BROAD DYNAMIC RANGE
Assay principle
The rabbit Fc kit is a competitive immunoassay for the measurement of rIgGs or any rFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to rFc and XL665 coupled rIgGs. rFc contained in the sample competes with XL665-labelled rabbit IgG for binding to anti-rabbit Fc Eu Cryptate.
Assay Protocol
The rabbit Fc assay features a streamlined protocol with only 1 incubation step after sample and Rabbit Fc detection reagents dispensing.
This protocol requires a single two-hour incubation period at RT.
Key features
| Rabbit Fc | |
|---|---|
| Detection limit: | 5 ng/mL |
| Dynamic range: | 4.9 80,000 ng/mL |
| Incubation time: | 2h at RT |
| EC50: | 178 ng/mL |
Product Insert Fc (rb) Kit / 6FRIGPEG
6FRIGPEG - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Safety Data Sheet Fc (rb) Kit / 6FRIGPEG
6FRIGPEG - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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