Rabbit Fc kit HTRF®

The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.


Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Rabbit Fc kit enables a rapid quantification of rabbit IgG and any proteins containing a rabbit Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.



Assay principle

The rabbit Fc kit is a competitive immunoassay for the measurement of rIgGs or any rFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to rFc and XL665 coupled rIgGs. rFc contained in the sample competes with XL665-labelled rabbit IgG for binding to anti-rabbit Fc Eu Cryptate.
Rabbit Fc kit assay principle

Assay Protocol

The rabbit Fc assay features a streamlined protocol with only 1 incubation step after sample and Rabbit Fc detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Rabbit Fc kit assay protocol

Key features

Rabbit Fc
Detection limit: 5 ng/mL
Dynamic range: 4.9 – 80,000 ng/mL
Incubation time: 2h at RT
EC50: 178 ng/mL
rabbit Fc standard curve

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Plate Reader Requirement

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