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SARS-Cov2 nucleocapsid detection kit HTRF®

This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

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This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

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Overview

The SARS-CoV-2 N protein is the basal monomeric constituent of the coronavirus oligomeric nucleocapsid. This protein is major immunogen predominantly expressed at early stages of viral infection and present from day one in infected patients’ serum. As such, SARS-CoV-2 N protein is a reliable marker for viral infection and a possible immunogen candidate for the development of a vaccine.

Detection of recombinant SARS-CoV-2 nucleocapsid

16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after 2h incubation at RT.
SARS-CoV-2 S1 protein standard curve

Standard curve Capsid kit

Sample size

16 µL
Final assay volume20 µL
Time to results2h at RT
Detection limit165 pg/mL
Dynamic range307 to 75,000 pg/mL
Sample compatibilityCell-based or cell supernatant
Nucleocpasid standard curve

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Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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