Home
Life sciences
GPCR platform
Labeled cells
Serotonin 5HT1AR expressing cells labeled with Terbium

Serotonin 5HT1AR expressing cells labeled with Terbium HTRF®

Tag-lite cells transiently expressing the Serotonin 5HT1A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

Fully functional cell line
Transient expression
Ready for binding

Download the protocols

Tag-lite cells transiently expressing the Serotonin 5HT1A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

Size

Cat. number selected :

Quantity

Overview

The 5-HT1A receptor is a GPCR widely distributed in regions that receive serotonergic input, including the frontal cortex, septum, amygdala, hippocampus, and hypothalamus. The serotonin 1A receptor (5-HT1A) plays a role in the genesis of several major depressive disorders.

Cells expressing the 5HT1A receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Benefits

PRE-LABELED WITH TERBIUM
FROZEN CELLS, READY TO BE USED
CELLS/LIGAND VALIDATED FOR BINDING

Assay principle

Running your 5HT1A receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Diagram of 5HT1A receptor binding assay using the Tag-lite protocol

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Diagram of a saturation binding assay using Tag-lite

Representative data obtained when running a saturation binding assay

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Diagram of a competitive binding assay using Tag-lite

Representative data obtained when running a competitive binding assay

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using Serotonin 5HT1A labeled cells and their matching fluorescent ligand (L0029RED). WAY-100135 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

Representative competitive binding graph

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

The easy way to approach biology without radioactivity - Flyers

Determination of association (kon) and dissociation (koff) rates constants using the Tag-lite platform

Challenge the limits of binding kinetics studies - Application Notes

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics

Available On-demand - Videos

Product Insert 5HT1A-R LabCell / C1TT15HT1A

C1TT15HT1A - Product Insert

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

GPCRs Pharmacology

A comprehensive overview of pharmacology's main ligands - Guides

GPCRs: the pathway to Discovery

Complete solutions for GPCR Drug Discovery - Brochures

Safety Data Sheet 5HT1A-R LabCell / C1TT15HT1A