Tag-lite SNAP(+) plasmid
Tag-lite SNAP(+) plasmid
SNAP-Lumi4-Tb labeling reagent HTRF®
SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. SNAP-Lumi4-Tb enables SNAP-tagged proteins to become labeled with a Terbium fluorophore.
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Overview
Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. SNAP-Lumi4-Tb allows the labeling of SNAP-Tagged proteins with a terbium fluorophore.
Benefits
- COVALENT LABELING
- FAST REACTION
Receptor labeling
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. SNAP-tag enables the specific and covalent labeling of any protein of interest. Cells are provided unlabeled and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available from the Cisbio catalog in 4 different sizes. For more details refer to labeling procedure.
Watch this video describing how to label cell surface receptors using Tag-lite® technology.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert SNAP-Tb Subst / SSNPTBC-SSNPTBD-SSNPTBG-SSNPTBX
SSNPTBC-SSNPTBD-SSNPTBG-SSNPTBX - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
Safety Data Sheet SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (DEU) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (ELL) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (FRA-FR) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (ITA) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (SPA) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (ENG-GB) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet (ENG-US) SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (DEU) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (ELL) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (FRA-FR) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (ITA) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (SPA) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (ENG-GB) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet (ENG-US) SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (ENG-US) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (DEU) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (ELL) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (FRA-FR) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (ITA) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (SPA) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet (ENG-GB) SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (DEU) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ELL) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (FRA-FR) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ITA) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (SPA) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ENG-GB) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ENG-US) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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