Tag-lite SNAP(+) plasmid
Tag-lite SNAP(+) plasmid
SNAP-Lumi4-Tb labeling reagent
SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. SNAP-Lumi4-Tb enables SNAP-tagged proteins to become labeled with a Terbium fluorophore.
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Overview
Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. SNAP-Lumi4-Tb allows the labeling of SNAP-Tagged proteins with a terbium fluorophore.
Benefits
- COVALENT LABELING
- FAST REACTION
Receptor labeling
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. SNAP-tag enables the specific and covalent labeling of any protein of interest. Cells are provided unlabeled and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available from the Cisbio catalog in 4 different sizes. For more details refer to labeling procedure.
Watch this video describing how to label cell surface receptors using Tag-lite® technology.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
Tag-lite binding assays
benefit from them to move away from radioactivity - Flyers
Determination of association (kon) and dissociation (koff) rates constants using the Tag-lite platform
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Webinar: The kinetics of drug-receptor binding: why it is important and how we can measure it
Featuring a panel of experts - Videos
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert SNAP-Tb Subst / SSNPTBC-SSNPTBD-SSNPTBG-SSNPTBX
SSNPTBC-SSNPTBD-SSNPTBG-SSNPTBX - Product Insert
Cisbio Product Catalog 2019
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet SNAP-Tb Subst / SSNPTBC
SSNPTBC - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBD
SSNPTBD - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBG
SSNPTBG - Safety Data Sheet
Safety Data Sheet SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (DEU) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ELL) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (FRA-FR) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ITA) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (SPA) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ENG-GB) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet
Safety Data Sheet (ENG-US) SNAP-Tb Subst / SSNPTBX
SSNPTBX - Safety Data Sheet