SNAP tag AT2 Angiotensin R cloned plasmid HTRF®
All information on this page pertains to the Tag-lite plasmid cloned with the AT2 Angiotensin receptor.
- FULL LENGTH RECEPTOR
- HIGH EXPRESSION SYSTEM
- VALIDATED IN EXPRESSION
Use standard transfection techniques (refer to transient transfection protocol) to transiently express the SNAP-GPCR of interest in your cell line.
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables the specific and covalent labeling of any protein of interest (refer to labeling procedure). Cells are provided unlabeled, and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available in 4 different pack sizes from the Cisbio catalog.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a set amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
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