Somatomedin C (IGF-I) immunoradiometric assay kit RIA
The IGF1-US kit is an immunoradiometric assay for a measure of somatomedin C (IGF-I).
For Research Use Only, not for use in diagnostic procedures.
- NO INTERFERENCE WITH BINDING PROTEINS
- NO EXTRACTION NECESSARY
Two monoclonal antibodies directed against two different antigenic sites of the IGF-I molecule are used for the sandwich. The first one is adsorbed onto the tube walls. The second one, radiolabeled with iodine-125, is used as the tracer. A predilution of the samples (1/31) with an acidic solution is required to strip the carrier protein (BP). This carrier protein is then saturated by IGF-II. The use of IGF-II molecules in the assay avoids any reassociation between IGF-I and the carrier protein (IGF-BP).
Assay procedure: 2h at RT / shaking + 2h at RT / shaking.
Working range: 1 – 1000 ng/mL (IRS 87/518)
Sample: serum or plasma – 25 µL - predilution required (1/31) Analytical sensitivity: 1 ng/mL