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Somatomedin C (IGF-I) immunoradiometric assay kit

Somatomedin C (IGF-I) immunoradiometric assay kit RIA

The IGF1-US kit is an immunoradiometric assay for a measure of somatomedin C (IGF-I).

For Research Use Only, not for use in diagnostic procedures.

The IGF1-US kit is an immunoradiometric assay for a measure of somatomedin C (IGF-I).

For Research Use Only, not for use in diagnostic procedures.

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Benefits

NO INTERFERENCE WITH BINDING PROTEINS
NO EXTRACTION NECESSARY

Assay principle: sandwich

Two monoclonal antibodies directed against two different antigenic sites of the IGF-I molecule are used for the sandwich. The first one is adsorbed onto the tube walls. The second one, radiolabeled with iodine-125, is used as the tracer. A predilution of the samples (1/31) with an acidic solution is required to strip the carrier protein (BP). This carrier protein is then saturated by IGF-II. The use of IGF-II molecules in the assay avoids any reassociation between IGF-I and the carrier protein (IGF-BP).

Assay procedure: 2h at RT / shaking + 2h at RT / shaking.